The Digby RCMP, Fire Department and paramedics responded to the crash at 7:20 p.m.
The car, driven by the deceased, had veered off the roadway and then struck a tree.
The woman was pronounced dead at the scene.
RCMP are continuing to investigate the crash with help from a collision re-constructionist.
Highway 217 is currently reduced to one lane between Timpany Lane and Gullivers Cove Road.
RCMP Staff Sergeant Jeff West said no specific factors have been determined relating to the crash.
Digby RCMP officers are still at the scene and will remain as the investigation continues.
